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Image Search Results
Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology
Article Title: Antibiotic exposure postweaning disrupts the neurochemistry and function of enteric neurons mediating colonic motor activity
doi: 10.1152/ajpgi.00088.2020
Figure Lengend Snippet: Primary and secondary antisera
Article Snippet:
Techniques:
Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology
Article Title: Antibiotic exposure postweaning disrupts the neurochemistry and function of enteric neurons mediating colonic motor activity
doi: 10.1152/ajpgi.00088.2020
Figure Lengend Snippet: Vancomycin (Vanco) exposure postweaning disrupted myenteric neuronal subtype proportions in the colon. A and B: representative images of myenteric plexus preparations from the midcolon of control- (Con) and Vanco-treated 6-wk-old mice. A: myenteric neurons were stained with the pan-neuronal marker Hu (red), calbindin (green), and neuronal nitric oxide synthase (nNOS; blue). Some calbindin-immunoreactive (closed arrowheads) and neuronal nitric oxide synthase (nNOS)-immunoreactive (open arrowheads) Hu+ neurons are indicated. Hu+ neurons that express both calbindin and nNOS (yellow arrowheads) are rare. B: myenteric neurons are stained for Hu (red), choline acetyltransferase (ChAT; green), and neurofilament-M (NFM; cyan). Some ChAT-immunoreactive (filled arrows) and NFM-immunoreactive (open arrows) Hu+ neurons are indicated. Some Hu+ neurons express both ChAT and NFM (yellow arrows). C: myenteric neuron (Hu+) density of the midcolon (n = 10–11 mice). D: quantification of nNOS+ (n = 10 mice), ChAT+ (n = 9–11 mice), calbindin+ (n = 10 mice) and NFM+ (n = 4–7 mice) neurons relative to Hu+ neurons in the mid colon. Data are represented as means ± SE. Treatment groups were compared statistically using unpaired t tests. *P < 0.05; **P < 0.01.
Article Snippet:
Techniques: Control, Staining, Marker
Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology
Article Title: Antibiotic exposure postweaning disrupts the neurochemistry and function of enteric neurons mediating colonic motor activity
doi: 10.1152/ajpgi.00088.2020
Figure Lengend Snippet: Vancomycin (Vanco) exposure postweaning disrupted submucosal neuronal subtype proportions in the colon. A–C: representative images of submucous plexus preparations from the mid-colon of control- (Con) and Vanco-treated 6-wk-old mice. A: submucosal neurons were stained with the pan-neuronal marker Hu (red), vasoactive intestinal peptide (VIP; green), and choline acetyltransferase (ChAT; blue). VIP-immunoreactive (closed arrowheads) and ChAT-immunoreactive (open arrowheads) Hu+ neurons are indicated. B: submucosal neurons are stained for Hu (red) and neurofilament-M (NFM; green). NFM-immunoreactive (filled arrowheads) Hu+ neurons are indicated. C: submucosal neurons are stained for Hu (red) and neuronal nitric oxide synthase (nNOS; green). nNOS-immunoreactive (filled arrowheads) Hu+ neurons are indicated. D: quantification of VIP+ and or ChAT+ neurons relative to Hu+ neurons in the midcolon (n = 4–5 mice). E: quantification of nNOS+, NFM+, and calbindin (Calb)/calretinin (Calr)+ neurons relative to Hu+ neurons in the mid-colon. Data are represented as means ± SE. Treatment groups were compared statistically using unpaired t tests. *P < 0.05; **P < 0.01.
Article Snippet:
Techniques: Control, Staining, Marker